THE IMMUNOSUPPRESSIVE EFFECT OF FRESH ALLOGENEIC BONE-GRAFT IN MICE

We investigated the immunosuppressive effect of fresh bone allografts after donor specific spleen cell transfusion in mice. Allografts from major histocompatibility complex incompatible mice were performed one week after the transfusion. Cellular and humoral immune responses were recorded by measuring alloreactive cytotoxic T-lymphocyte activity an d antibody activity. Survival in days of a second set skin graft was m easured for determining clinical immunity. Alloreactive cytotoxic T-ly mphocyte activity was stimulated at 17 days (10 days after bone grafti ng) and then suppressed at 31 days (24 days after bone grafting). The survival of the second set skin graft was not diminished at this time. This outcome indicates that the spleen cell stimulated cellular immun e response was suppressed after fresh bone allografts, suggesting that these allografts have an immunosuppressive effect and induce immunosu ppressive actions.