Gestational choriocarcinoma whose responsible pregnancy was a complete hydatidiform mole identified by PCR analysis with new sequence tagged site primers

We report a case where the pregnancy responsible for a gestational chorioca rcinoma was not the antecedent pregnancy or the second normal term delivery , but a complete hydatidiform mole that had advanced to clinically invasive mole. This responsible pregnancy was identified by polymerase chain reacti on analysis (PCR). PCR analysis was performed by using five new sets of seq uence-tagged site (STS) primers on four chromosomes (chr. 1, D1S225; chr. 3 , D3S1744; chr. 12, D12S1090; chr. 18, D18S849 and D18S877). The constituti on of alleles of choriocarcinoma was shown to be almost identical with that of the husband on every marker. The allele patterns of choriocarcinoma on D3S1744 and D12S1090 were not observed with DNA from the patient. The band pattern originating from molar DNA was also identical with those of the hus band and choriocarcinomas an D18S849 and D1S225.