Sophorolipid production by Candida bombicola: Medium composition and culture methods

Citation
Ja. Casas et F. Garcia-ochoa, Sophorolipid production by Candida bombicola: Medium composition and culture methods, J BIOSCI BI, 88(5), 1999, pp. 488-494
Citations number
21
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
88
Issue
5
Year of publication
1999
Pages
488 - 494
Database
ISI
SICI code
1389-1723(199911)88:5<488:SPBCBM>2.0.ZU;2-3
Abstract
Candida bombicola is able to produce sophorolipid molecules with surfactant properties when grown in a medium composed of two different carbon sources (usually sugar and oil) and a nitrogen source (frequently yeast extract). In this work, the composition of the medium and the culture method employed have been studied. The influences of glucose concentration, properties of oil and yeast extract concentration have been taken into account. According ly, a production medium composition is proposed (100 g/l glucose, 100 g/l s unflower oh and I g/l yeast extract). The most frequent culture methods rep orted in Literature, i.e. batch, medium pulse mode and resting-cell methods , have been tested. The resting-cell method was found to produce the highes t final concentration of sophorolipid, obtaining a good yield of carbon sou rces in a relatively short time. Under the best operational conditions and using the resting-cell method, 120 g/l sophorolipid was obtained in 8 d, wi th a carbon source yield of 0.60. Product distribution has also been invest igated and the sophorolipid molecular structure of opened or cycled molecul es has been determined under different operational conditions. Low yeast ex tract concentration and long fermentation time enhance the production of cy cled structures by all the production methods studied.