Ba. Odland et al., Quality control guidelines for disk diffusion and broth microdilution antimicrobial susceptibility tests with seven drugs for veterinary applications, J CLIN MICR, 38(1), 2000, pp. 453-455
This multicenter study proposes antimicrobial susceptibility (MIC and dish
diffusion methods) quality control (QC) parameters for seven compounds util
ized in veterinary health. Alexomycin, apramycin, tiamulin, tilmicosin, and
tylosin were tested by broth microdilution against various National Commit
tee for Clinical Laboratory Standards (NCCLS)-recommended QC organisms (Sta
phylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Streptococ
cus pneumoniae ATCC 49619, Escherichia coli ATCC 25922, and Pseudomonas aer
uginosa ATCC 27853). In addition, disk diffusion zone diameter QC limits we
re determined for apramycin, enrofloxacin, and premafloxacin by using E. co
li ATCC 25922, P. aeruginosa ATCC 27853, and S. aureus ATCC 25923. The resu
lts from five or six participating laboratories produced greater than or eq
ual to 99.0% of MICs and greater than or equal to 95.0% of the zone diamete
rs,within suggested guidelines. The NCCLS Subcommittee for Veterinary Antim
icrobial Susceptibility Testing has recently approved these ranges for publ
ication in the next M31 document.