M. Balcells et al., Quantitative assessment of protein adsorption by combination of the enzyme-linked immunosorbent assay with radioisotope-based studies, J COLL I SC, 220(2), 1999, pp. 198-204
Protein adsorption at polymer surfaces has been investigated by means of bo
th ELISA and radiolabeling techniques. Most of the data obtained are linear
ly related to each other for protein concentrations between 0.01 and 1 mu g
/ml, i.e., the concentration range in which the maximum amount of adsorbed
active protein (ELISA) is achieved. The correlation of ELISA data with radi
oisotope-based measurements allows quantification of the former. Specific c
orrelation factors are described. Adsorption is shown to be strongly depend
ent an the polymer/protein system. (C) 1999 Academic Press.