Determination of 2,6-pyridinedicarboxylic acid in peach juice

The quantitation of 2,6-pyridinedicarboxylic acids (2,6-PDA) in fruit juice can be achieved rapidly utilizing High Pressure Liquid Chromatography (HPL C). The best chromatographic performance was obtained with an aqueous mobile ph ase containing 15 mM tetrabutylammonium phosphate and 2 mM EDTA; the pH was maintained at 7.2 by 10 mM phosphate buffer. The influence of the eluent parameters on retention of PDAs has been invest igated in order to elucidate the separation mechanisms involved in the ion pair chromatography of these ionizable substances.