A collection of 332 PVY isolates, originating from potato crops. was charac
terized in order to seek the newly described PVY variants, (PVYW)-W-N and P
VYZ Serologically, 284 samples were found to contain only PVYO, 36 samples
to contain only PVYN, whereas 12 samples were doubly infected by both PVYO
and PVYN. All 36 isolates, serologically PVYN-typed and 16 isolates serolog
ically PVYO-typed, induced vein necrosis on tobacco. The latter 16 isolates
reacted like a Polish standard (PVYW)-W-N isolate and are probably new mem
bers of that PVY subgroup; in the panel of samples, they originated from po
tato cultivars never previously reported as infected by (PVYW)-W-N and thei
r high incidence probably indicates that they are more widespread than comm
only accepted. Indexing of 108 PVYO isolates on three potato cultivars (Des
iree, Eersteling and Maris Bard) carrying hypersensitivity resistance genes
to PVYO, PVYC and PVYZ, respectively, revealed the presence of various pat
hotypes. Two isolates reacted as two standard isolates, designated PVYZE, O
riginating from Spain and previously suspected to be PVYZ members; these is
olates were able to overcome the hypersensitivity genes in the three potato
cultivars and consequently were members of a newly recognized pathotype. A
nother isolate evoked the reactions expected after infection with PVYZ Surp
risingly, PVYC was only found in one sample that was doubly infected by PVY
O.