Suppression of cell metabolism by structuring water under hydrophobic-gas pressure

We propose a new method to suppress cell metabolism by dissolving hydrophob ic gas into intracellular water, and structuring it under hydrophobic-gas p ressure. Xenon, krypton, argon, and helium gases show death inhibitory effe cts on all test bacteria (Escherichia coli, Pseudomonas aeruginosa, Bacillu s: subtilis, Staphylococcus aureus and Streptococcus salivarius) suspended in physiological saline with no nutrient component during storage under the conditions. In addition, different death inhibitory effects are observed a mong the five test bacteria, and this proposed method is more effective for gram-negative bacteria with thin cell wails than for gram-positive bacteri a with thick cell walls. Further, xenon gas exhibit more significant effect s for death inhibition than any other test gases, and the optimum initial p artial pressure to inhibit the death is observed only for xenon gas in the present experimental conditions. Consequently, it is experimentally suggest ed that the cell metabolism can be suppressed almost perfectly by making th e optimum water structure.