Background and Purpose: Serologic testing for antibody to monkey B virus (B
V) in macaque sera is problematic due to the biohazardous nature of BV and
BV antigens, Herpesvirus papio 2 (HVP2), a herpesvirus of baboons, is more
closely related genetically and antigenically to BV than is human herpes si
mplex virus 1 (HSV1), The potential for use of HVP2 relative to HSV1 as an
alternative test antigen for detection of anti-BV antibody in macaque sera
was assessed.
Methods: Standard ELISA formats mere developed, using BV-, HVP2-, and HSV1-
infected cell extracts. Performance of the HVP2 and HSV1 tests was assessed
relative to that of the BV test.
Results: Using the BV antigen ELISA, 349 sera from 7 macaque species were t
ested, and results were classified as positive (253), negative (94), or sus
pect (2), The ELISA using HVP2 antigen detected 98.0% of BV-positive sera (
248 of 253), whereas the HSV1-based ELISA detected only 96.0% (243 of 253),
All three ELISAs identified the same two samples as suspect, and the HSV1
ELISA identified three additional BV-positive sera as suspect.
Conclusions: The HVP2 antigen-based ELISA was equal in sensitivity and spec
ificity to the BV antigen-based ELISA and was superior to the HSV1 ELISA fo
r detection of BV-positive macaque sera. In addition, the HVP2 ELISA has gr
eater laboratory safety, compared with BV antigen use for ELISA testing.