Effect of decaglycerol mono-oleate on chemiluminescence of human neutrophils

Decaglycerol mono-oleate (DGMO), a type of food additive (a polyglycerol es ter of fatty acids), was evaluated for its in vitro effect on reactive oxyg en species (ROS),generation by human neutrophils using a luminol-dependent chemiluminescence assay. Neutrophils were isolated by the Histopaque-1077/1 119 double density gradient technique. Opsonized zymosan (OZ) and phorbol 1 2-myristate 13-acetate (PMA) were employed as artificial stimuli. DGMO inhi bited the chemiluminescence response of OZ-stimulated neutrophils by 58% (p < 0.01) at a concentration of 0.5 mg/mL. DGMO inhibited the PMA-induced ch emiluminescence by 40% (p < 0.01) at a concentration of 0.01 mg/mL. The inh ibition phenomena could not be attributed to light absorption by DGMO alone , because the percentage chemiluminescence in the presence of DGMO is lower than the percentage transmittance of DGMO at 425 nm. These results suggest that DGMO downregulates ROS generation by human neutrophils. Copyright (C) 1999 John Wiley & Sons, Ltd.