Effects of newly synthesized analogs of MIF-1 containing unnatural amino acids on electrically evoked smooth muscle contractions

New MIF-I (Pro-Leu-Gly-NH2) analogs containing unnatural amino acids such a s L-canavanine (Cav) and L-cysteic acid S-(2-aminoethyl)amide (sLys) have b een synthesized and in vitro experiments were performed to study their acti on on neurotransmission in target tissues with adrenergic and cholinergic n eurotransmission. The experiments were carried out on electrically stimulat ed proximal guinea pig ileum (GPI) and the prostatic part of rat and rabbit vasa deferentia (VDR, VDRaBB). The present results show that the newly syn thesized Cav(2)-MIF and sLys(2)-MIF might affect electrically evoked smooth muscle contractions. (C) 1999 Prous Science. All rights reserved.