CPR-total (TAFI and activated TAFI) levels in plasma/serum of hemophiliacs

Arginine carboxypeptidase (CPR) is a single-chain plasma protein generated during coagulation from a precursor (proCPR), proCPR is the same molecule a s thrombin activable fibrinolysis inhibitor (TAFI), which retards fibrin cl ot lysis in vitro and most likely modulates fibrinolysis in vivo. In this s tudy, the amount of CPR-total, which includes proCPR (TAFI) and CPR (activa ted TAFI), in hemophiliac patients was evaluated using a newly developed en zyme linked immunosorbent assay (ELISA). The amount of CPR-total in plasma or serum of most of the hemophiliac patients was in the range of healthy in dividuals. There was no significant difference in hemophiliac patients with or without HIV-1 infection. However two out of the 74 hemophiliac patients shelved a significantly high level. The upregulation of CPR-total might co ntribute to compensate for inefficient coagulation in some hemophiliac indi viduals.