Age-dependent changes in lipid peroxide levels in peripheral organs, but not in brain, in senescence-accelerated mice

The tissue concentration of lipid peroxides was determined in the brain, he art, liver, lung and kidney of accelerated senescence-prone (SAMP-8) and -r esistant (SAMR-1) mice at 3, 6 and 9 months of age by a method involving ch emical derivatization and high performance liquid chromatography. The level of lipid peroxides in the brain did not show an age-dependent change, but at each age the brain level of lipid peroxides was significantly higher in SAMP-8 than in SAMR-1. In contrast, the lipid peroxide levels in the periph eral organs showed increases with aging in both strains, and they were sign ificantly higher in SAMP-8 than in SAM R-1 at both 3 and 6 months of age (e xcept at 3 months of age in the kidney). These results suggest that increas ed oxidative stress in the brain and peripheral organs is a cause of the se nescence-related degeneration and impairments seen in SAMP-8. (C) 2000 Else vier Science Ireland Ltd. All rights reserved.