Natural selection plays a fundamental role in most theories of speciation,
but empirical evidence from the wild has been lacking. Here the post-Pleist
ocene radiation of threespine sticklebacks was used to infer natural select
ion in the origin of species. Populations of sticklebacks that evolved unde
r different ecological conditions show strong reproductive isolation, where
as populations that evolved independently under similar ecological conditio
ns lack isolation. Speciation has proceeded in this adaptive radiation in a
repeatable fashion, ultimately as a consequence of adaptation to alternati
ve environments.