Identification of melon cultivars by gel and capillary electrophoresis

Citation
R. Bonfitto et al., Identification of melon cultivars by gel and capillary electrophoresis, SEED SCI T, 27(2), 1999, pp. 779-783
Citations number
5
Categorie Soggetti
Plant Sciences
Journal title
SEED SCIENCE AND TECHNOLOGY
ISSN journal
02510952 → ACNP
Volume
27
Issue
2
Year of publication
1999
Pages
779 - 783
Database
ISI
SICI code
0251-0952(1999)27:2<779:IOMCBG>2.0.ZU;2-Z
Abstract
Capillary electrophoresis (CE) in conjunction with sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE) was used to identify nine cul tivars of melons. The identification was based on qualitative differences s hown by seed storage proteins (globulins) separated by the two techniques. Although CE showed good reproducibility, it failed to identify all of the c ultivars examined. The use of CE in conjunction with SDS-PAGE increased the possibility of cultivar identification. The inability of these techniques to discriminate between all of the nine cultivars examined might be due to the rather conservative nature of the globulins extracted from the same mem bers of the Cucurbitaceae family.