A genetic map of Maritime pine based on AFLP, RAPD and protein markers

The AFLP (amplified fragment length polymorphism) technique was adapted to carry out genetic analysis in maritime pine, a species characterized by a l arge genome size (24 pg/C). A genetic linkage map was constructed for one F -1 individual based on 239 AFLP and 127 RAPD (randomly amplified polymorphi c DNA) markers. Markers were scored on megagametophytes (In) from 200 germi nated F-2 seedlings. Polymorphism rate, labour time and cost of both AFLP a nd RAPD techniques were compared. The AFLP technique was found to be twice as fast and three-times less costly per marker than the RAPD technique. Thi rteen linkage groups were identified with a LOD score greater than or equal to 6 covering 1873 cM, which provided 93.4% of genome coverage. Proteins w ere extracted from needles (2n) of the F-2 progeny and revealed by 2-DE (tw o-dimensional electrophoresis). Thirty one segregating proteins were mapped using a QTL detection strategy based on the quantification of protein accu mulation. Two framework maps of the same F-1 individual are now available. The first map (Plomion et al. 1996) uses RAPD markers and the second map, p resented in this study, uses mostly AFLP markers. Although the total geneti c length of both maps was almost identical, differences among homologous gr oups were observed.