The study on the reaction between 3-[di(carboxymethyl)aminomethyl]-1,2-dihydroxyanthraquinone and bovine serum albumin

The interaction of 3 - [di (carboxymethyl) aminomethyl] - 1 , 2 - dihydroxy anthraquinone (AFB) and bovine serum albumin (BSA) was investigated by spec trophotometric method in acidic solution (pH4 . 1). When BSA was added into AFB solution, a purple color was observed, which indicated formation the o f the AFB - BSA complex. The maximum absorption of the complex was obtained at 510nm, with the apparent molar absorptivity of epsilon = 1.2474 x 10(4) mol (- 1) . cm (- 1) . L. Two conditional constants were defined: the maxim um binding number n = 7 and apparent binding constant K-C = 3.85 x 10(6). I t is suggested that the electrostatic force is the main binding feme. The S catchard model is appropriate in the treatment of data obtained here. It is found that sodium chloride concentration of the solution has significant e ffect on the sensitivity of AFB - BSA complex.