The widespread presence of adenoviruses in various species makes it probabl
e that a carrier state and infection also exist in cars; this could be prov
ed earlier. On the basis of these considerations investigations were carrie
d out to perform an epidemiological survey in 100 randomly selected feline
sera from the Czech Republic. Antibody detection was carried out in a group
-specific indirect ELISA employing purified adenovirus hexon antigen. The a
denovirus seropositivity was found to be 25% among the samples tested. Dome
stic short-haired cats (28%)and females (34%) showed increased infection ra
tes. Among the 6 retrovirus-infected cats 3 males with outdoor access were
seropositive. Cats showing respiratory and gastrointestinal symptoms gave h
igh prevalence. These results are similar to those found in other species a
nd feline populations and reflect on the natural adenovirus infection of ca
ts in the Czech Republic.