C. Boudreau-lariviere et al., Myotubes originating from single fast and slow satellite cells display similar patterns of AChE expression, AM J P-REG, 278(1), 2000, pp. R140-R148
Citations number
45
Categorie Soggetti
Physiology
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
Slow- and fast-contracting skeletal muscles of both rats and mice display s
ignificant differences in their patterns of acetylcholinesterase (AChE) exp
ression. Although neural influences are known to account for a large propor
tion of these differences, intrinsic variations between fast and slow myoge
nic precursor cells have been implicated. In the present study, we have cap
italized on the use of Immorto transgenic mice to obtain single myogenic pr
ecursor cells isolated from either slow or fast muscle fibers and determine
d whether these cells generated myotubes that produced distinct patterns of
AChE expression as observed in vivo between slow and fast muscles. These t
wo myotube populations displayed similar cell-associated and secreted AChE
enzyme activity as well as comparable levels of AChE transcripts. Both myot
ube populations also expressed nearly identical molecular form profiles. By
contrast, AChE activity and transcript levels were approximately two- and
fivefold greater in fast skeletal muscles compared with slow ones. Together
, these findings indicate that differences in AChE expression between fast
and slow muscles are not due to inherent differences in myogenic precursor
cells, thereby suggesting that other factors, such as innervation, play a p
redominant role in establishing the distinct patterns of AChE expression in
these muscle types.