Age-related progressive renal fibrosis in rats and its prevention with ACEinhibitors and taurine

Our previous studies demonstrated an increased reactive oxygen species (ROS ) production, as well as transforming growth factor-beta 1 (TGF-beta 1) exp ression in the rat kidney with aging. In the present study, we examined the effect of aging on extracellular matrix (ECM) accumulation and the effects of treatment with angiotensin-converting enzyme inhibitors (captopril and lisinopril) and taurine, an antioxidant amino acid. Age-related increases i n types I and IV collagen and fibronectin mRNA expression were found at 24 and 30 mo of age. In contrast, type III collagen only increased in 30-mo-ol d rats. Captopril-, lisinopril-, and taurine-treated animals showed a stati stically significant decrease in ECM protein expression at both ages. Moreo ver, treatment with taurine reduced the TGF-beta 1 mRNA levels in 24- and 3 0-mo-old rats by 40%. Taurine also completely blocked increases in type I a nd type IV collagen expression in mesangial cells in response to TGF-beta 1 . Our results demonstrate a protective role from both converting enzyme inh ibitors and taurine in the age-related progressive renal sclerosis. In addi tion, taking into account that taurine is considered as an antioxidant amin o acid, present data suggest a role for ROS in age-related progressive rena l fibrosis, perhaps through interactions with the TGF-beta 1 pathway.