Expression of different chemokines by human osteosarcoma cells in responseto tumor necrosis factor-alpha

We investigated the expression of different chemokines in the surnatants an d inside the cells of four human osteosarcoma cell lines. HOS, U-2 OS, MG63 and Saos-2 cells were cultured for 24, 48, 72 hours both in basal conditio ns and after stimulus with TNF alpha. Human stromal cells were used as cont rol. IL-8 and MCP-I are present in higher concentration in the surnatants i n contrast to RANTES which is present primarily inside the cells. IL-8 and MCP-1 are not totally expressed by all the human osteosarcoma cell lines in unstimulated conditions, but became detectable after TNF alpha treatment. In general, this cytokine stimulated the production and release of the thre e chemokines.