Angiography of fluoresceinated anti-vascular endothelial growth factor antibody and dextrans in experimental choroidal neovascularization

Citation
Mj. Tolentino et al., Angiography of fluoresceinated anti-vascular endothelial growth factor antibody and dextrans in experimental choroidal neovascularization, ARCH OPHTH, 118(1), 2000, pp. 78-84
Citations number
25
Categorie Soggetti
Optalmology,"da verificare
Journal title
ARCHIVES OF OPHTHALMOLOGY
ISSN journal
00039950 → ACNP
Volume
118
Issue
1
Year of publication
2000
Pages
78 - 84
Database
ISI
SICI code
0003-9950(200001)118:1<78:AOFAEG>2.0.ZU;2-6
Abstract
Objective: To determine if anti-vascular endothelial growth factor antibody and a range of dextrans with varying diffusion radii and molecular weights are permeable through experimental choroidal neovascularization (CNV). Methods: Choroidal neovascularization was induced in 10 cynomolgus monkey r etinas by means of argon laser injury. Digital fundus fluorescein angiogram s were performed with fluorescein sodium: fluoresceinated IgG antibodies (a nti-vascular endothelial growth factor and a control antibody), and fluores ceinated dextrans with molecular weights of 4, 20, 40, 70 and 150 kd. The 4 0- and 70-kd dextrans straddle the effective diffusion radius of IgG. For e ach reagent, early and late angiograms were performed in a standardized fas hion, with follow-up images obtained to monitor residual fluorescence. Results: Perfusion of retinal vessels and choroidal vasculature was seen wi th all reagents. Fluorescein and 4-and 20-kd dextran leaked rapidly from th e CNV within the first minute. Angiography with the use of 40-kd dextran an d fluoresceinated antibody, either anti-vascular endothelial growth factor or control IgG, showed fluorescence within the CNV that increased during th e first 1 to 5 hours, with mild leakage from the CNV. By 24 hours, fluoresc ence in the CNV was minimal, although in some cases persistent fluorescence in the surrounding tissue was evident up to 2 weeks. The 70-kd dextran sho wed fluorescence within the CNV and leakage in 1 of 3 eyes, The 150-kd dext ran showed fluorescence within the CNV but did not demonstrate leakage. Conclusions: Fluoresceinated antibodies and dextran with smaller effective diffusion radii showed CNV perfusion and leakage. Dextrans with larger effe ctive diffusion radii (70 kd and 150 kd) perfused into CNV but did not show leakage consistently. Clinical Relevance: Determining the permeablity of antibodies and molecules of similar size through CNV can help ascertain the feasibility of using in travenously administered antibodies against angiogenic growth factors as a future treatment for choroidal neovascularization.