Comparison of the complete sequences of five different isolates of Potato virus A (PVA), genus Potyvirus

The complete nucleotide (nt) and deduced amino acid (aa) sequences of isola tes Ali, U, Her (from potato, Solanum tuberosum) and TamMV (from tamarillo, Solanum betacea) of Potato virus A (PVA, genus Polyvirus) were determined and compared with the previously reported sequence of PVA isolate B11. Most parts (proteins) of the polyprotein showed over 95% aa sequence similarity . The cylindrical inclusion (CI) protein and the 6K1 protein were the most conserved proteins among the five isolates. TamMV was the most different is olate. Sequence similarity between TamMV and the other isolates was the low est in regions close to the 5'-end [5'-non-translated region (NTR) and P1 r egion] and 3'-end (N-terminus of coat protein) of the genome. However, the termini of the genome (the first 60 nt of the 5'-NTR and the entire 3'-NTR) were highly similar in all five isolates. A frameshift region in the repli case (NIb) was identified the PVA isolates Ali, B11, Her and U, as compared to TamMV and other potyviruses.