Og. Shcherbakova et Mv. Filatov, Camptothecin enhances random integration of transfected DNA into the genome of mammalian cells, BBA-MOL CEL, 1495(1), 2000, pp. 1-3
Citations number
14
Categorie Soggetti
Cell & Developmental Biology
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
In order to study the involvement of DNA topoisomerase I (top1) in recombin
ation, we examined the effect of the antineoplastic drug camptothecin, whic
h selectively poisons top1 by trapping top1-cleavable complexes on integrat
ion of exogenic vector into the genome of mammalian cells. We transfected m
ouse F9 teratocarcinoma cells as well as Chinese hamster V79 cells with a p
lasmid carrying a selectable neo gene treated with camptothecin, and determ
ined the frequency of neo+ (G418(R)) colonies. We found that treatment with
camptothecin for as short a time as 4 h after electroporation resulted in
a 4- to 33-fold stimulation of plasmid integration into the recipient genom
e via non-homologous recombination. These results imply that top1-cleavable
complexes trapped by camptothecin could be potentially recombinogenic stru
ctures and could stimulate non-homologous recombination in vivo, promoting
the integration of transfected plasmids into mammalian genome. (C) 2000 Els
evier Science B.V. All rights reserved.