Preservation, mass production and storage of Stagonospora convolvuli, a bioherbicide candidate for field bindweed (Convolvulus arvensis)

Citation
Ha. Pfirter et al., Preservation, mass production and storage of Stagonospora convolvuli, a bioherbicide candidate for field bindweed (Convolvulus arvensis), BIOCONTROL, 44(4), 1999, pp. 437-447
Citations number
21
Categorie Soggetti
Entomology/Pest Control
Journal title
BIOCONTROL
ISSN journal
13866141 → ACNP
Volume
44
Issue
4
Year of publication
1999
Pages
437 - 447
Database
ISI
SICI code
1386-6141(1999)44:4<437:PMPASO>2.0.ZU;2-H
Abstract
Different solid substrates were investigated as spore production methods fo r Stagonospora convolvuli strain LA39, a potential bioherbicide for field b indweed (Convolvulus arvensis L.). Up to 4 x 10(8) spores/g of substrate we re yielded on cous-cous (cracked hard wheat). The spores were as pathogenic as those grown on artificial medium (V-8-juice agar). The air-drying on ka olin and storage at 3 degrees C kept spores viable and pathogenic for 180 d ays. Spore germination exceeded 70% for the first 140 days and then decline d to 50% after 175 days. Less than 5% of spores were still viable after 17 months. The preservation of stock cultures in 10% glycerine at -80 degrees C and in liquid nitrogen did not affect viability or pathogenicity of the s pores.