This study evaluated the effect of cultivation and reseeding on the distrib
ution and fate of soil mineral N (SMN), soluble organic N (SON) and potenti
ally mineralisable N (PMN) in the soil profile of two longterm grasslands i
n the UK. Cultivation and reseeding significantly increased the total solub
le N concentration (SMN plus SON) of the soil profile (0-90 cm), with over
50 mg SON kg(-1) observed. By contrast, the PMN pool was unaffected by cult
ivation and declined with increasing soil depth. The flush in SON and SMN o
bserved in both soils disappeared within 1 year following cultivation. The
fate of SON appeared to be dependent on soil type, with considerably more m
ovement to deeper layers apparent in the profile of a silty clay loam (30%
clay) than in a clay loam (49% clay). Mineralisation and/or immobilisation
of SON in the topsoil probably accounted for the changes observed in the SO
N content of the clay learn. SON is an important N pool in grassland soils
and cultivation has a significant impact on its release. Measurements of SO
N Should therefore be included in studies of N cycling in agricultural crop
ping systems, so that full account may be taken of its potential as a sourc
e or sink of mobile N.