A simple method for monitoring chromatography column eluates for laccase activity during enzyme purification

Authors
Dekker, RFH Ling, KY Barbosa, AM
Citation
Rfh. Dekker et al., A simple method for monitoring chromatography column eluates for laccase activity during enzyme purification, BIOTECH LET, 22(2), 2000, pp. 105-108
Citations number
8
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
2
Year of publication
2000
Pages
105 - 108
Database
ISI
SICI code
0141-5492(200001)22:2<105:ASMFMC>2.0.ZU;2-Q
Abstract
A simple and economical method is described that allows rapid detection of laccase activity in chromatography column fractions during enzyme purificat ion. Aliquots of column eluants are applied to filter paper coated with 2,2 '-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) containing a numb ered grid, and incubated at ambient temperature for 20 min. Indications of enzyme activity are simply observed by a colour change. This method avoids having to manually assay each fraction of a chromatographic run for enzyme activity.