Crown ether activates cholesterol oxidase in low water media

Authors
Sakodinskaya, IK Ryabov, AD
Citation
Ik. Sakodinskaya et Ad. Ryabov, Crown ether activates cholesterol oxidase in low water media, BIOTECH LET, 22(2), 2000, pp. 173-176
Citations number
20
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
2
Year of publication
2000
Pages
173 - 176
Database
ISI
SICI code
0141-5492(200001)22:2<173:CEACOI>2.0.ZU;2-B
Abstract
Kinetic studies of cholesterol oxidase-catalysed oxidation of cholesterol i n water/2-propanol mixtures showed a decrease of V-max/K-m values on the in crease of concentration of the organic co-solvent. Addition of 18-crown-6 t o the reaction medium results in an increase of V-max up to 16 times, and V -max/K-m up to 8.4 times, enhancing the activity of cholesterol oxidase in 2-propanol/water (88:12 v/v) to 3.5 times compared to the level observed in 46% 2-propanol.