Efficient secretion of human endostatin in the yeast, Pichia pastoris

Authors
Duan, JB Cai, X Zhang, BL Li, YZ Zou, MJ Wang, JX
Citation
Jb. Duan et al., Efficient secretion of human endostatin in the yeast, Pichia pastoris, BIOTECH LET, 21(12), 1999, pp. 1095-1099
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
21
Issue
12
Year of publication
1999
Pages
1095 - 1099
Database
ISI
SICI code
0141-5492(199912)21:12<1095:ESOHEI>2.0.ZU;2-E
Abstract
Endostatin is a 20 kDa COOH-terminal fragment of collagen XVIII that inhibi ts angiogenesis and tumor growth. The cDNA coding for human endostatin in h uman fetal liver has been cloned into the secreting expression organism Pic hia pastoris, and the high level expression of human endostatin has been ac hieved (about 200 mg of endostatin in 11 of culture). The recombinant human endostatin was purified to homogeneity by heparin-affinity column, and sho wed antiproliferative effect on rat brain micro-vascular endothelia cells.