Two streptokinase genes are expressed with different solubility in Escherichia coli W3110

Authors
Pupo, E Baghbaderani, BA Lugo, V Fernandez, J Paez, R Torrens, I
Citation
E. Pupo et al., Two streptokinase genes are expressed with different solubility in Escherichia coli W3110, BIOTECH LET, 21(12), 1999, pp. 1119-1123
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
21
Issue
12
Year of publication
1999
Pages
1119 - 1123
Database
ISI
SICI code
0141-5492(199912)21:12<1119:TSGAEW>2.0.ZU;2-5
Abstract
The streptokinase (SK) gene from S. equisimilis H46A (ATCC 12449) was clone d in E. coli W3110 under the control of the tryptophan promoter. The recomb inant SK, which represented 15% of total cell protein content, was found in the soluble fraction of disrupted cells. The solubility of this SK notably differed from that of the product of the SK gene from S. equisimilis (ATCC 9542) which had been cloned in E. coli W3110 by using similar expression v ector and cell growth conditions, and occurred in the form of inclusion bod ies.