ss-DNA immobilization and hybridization on nano-gold/2-aminoethane thoil/glassy carbon electrode

Citation
Q. Miao et al., ss-DNA immobilization and hybridization on nano-gold/2-aminoethane thoil/glassy carbon electrode, CHEM J CH U, 21(1), 2000, pp. 27-30
Citations number
5
Categorie Soggetti
Chemistry
Journal title
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN journal
02510790 → ACNP
Volume
21
Issue
1
Year of publication
2000
Pages
27 - 30
Database
ISI
SICI code
0251-0790(200001)21:1<27:SIAHON>2.0.ZU;2-A
Abstract
The modification of glassy carbon electrode (GCE) with nano-gold(NG) by che mical adsorption of 2-aminoethane thoil (AET) was achieved for the first ti me. On this electrode 15-mer ss-DNA was immobilized, then ss-DNA/NG/AET/GCE was formed. By using Co(bpy)(3)(3+), hybridization events of the immobiliz ed ss-DNA was well detected. It was found that the NG could possibly cause a parr of the ss-DNA to denature and bind Co(bpy)(3)(3+). However soaking i n pH 7.0 phosphate buffer solution, denaturization can be avoided and recog nition ability of hybridization was enhanced. Cyclic voltammetric behavior of Co(bpy)(3)(3+/2+) bound to the ds-DNA/NG/AET/GCE was studied. It was fou nd that the peak current(i(p)) showed a linear dependence on the potential scan rate up to 80 mV/s. It was demonstrated that GCE modified with NG by t his method had higher stability and reliability.