Analysis of biologically active compounds in Chinese medicines by molecular biochromatography with human serum albumin (HSA) stationary phase

A strategy for analysis of bioactive compounds in traditional Chinese medic ine was first proposed by molecular biochromatography with human serum albu min(HSA) stationary phase. Separation of active compounds in four kinds of traditional Chinese medicine(TCM) was compared on the HSA column, and Pound that different TCM show different charateristic of chromatogram patterns, The effects of extracting solutions( water/methanol/ethanol) and extracted rime on the amount of bioactive compounds in Angelica sinensis(Oliv.) Diels were examined, and a quality control method was also developed based on th is technique, which is able to simultaneously determine the concentrations of ferulic acid and liguistilide in extracted solutions of Angelica sinensi s(Oliv..) Diels by water and methanol.