Immobilization of STI on polymerized liposome by inter arm and its affinity adsorption for trypsin

Authors
Zhang, WT Yu, K Sun, Y
Citation
Wt. Zhang et al., Immobilization of STI on polymerized liposome by inter arm and its affinity adsorption for trypsin, CHEM J CH U, 21(1), 2000, pp. 62-65
Citations number
12
Categorie Soggetti
Chemistry
Journal title
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN journal
02510790 → ACNP
Volume
21
Issue
1
Year of publication
2000
Pages
62 - 65
Database
ISI
SICI code
0251-0790(200001)21:1<62:IOSOPL>2.0.ZU;2-Z
Abstract
Soybean trypsin inhibitor(STI) was immobilized on polymerized liposome of p entacose-10, 12-diene-1-ol-phospho ethanolamine by diglycolic anydride, glu taraldehyde and it 1,4-butanediol diglycidyl ether. In the course of precip itation, 20% to 60% trypsin does cleavage from PL-STI and shows PL-STI can be used as the affinity precipitation adsorbent of enzyme.