Two-enzyme system for the synthesis of 1-lauroyl-rac-glycerophosphate (lysophosphatidic acid) and 1-lauroyl-dihydroxyacetonephosphate

A combination of two enzymes, phospholipase D (PL D) and C (PL C), was inve stigated for the production of two lysophospholipids, 1-lauroyl-rac-glycero phosphate (1-LGP) and 1-lauroyl-dihydroxyacetonephosphate (1-LDHAP). The hi gh transphosphatidylation ability of phospholipase D from Streptomyces sp. allowed the formation of 1-lauroyl-phosphatidylglycerol (1-LPG) and 1-lauro yl-phosphatidyldihydroxyacetone (1-LPDHA) from phosphatidylcholine (PC) and 1-monolauroyl-rac-glycerol (1-MLG) and 1-lauroyl-dihydroxyacetone (1-MDHA) , respectively. A two-phase system, diethyl ether/water, was chosen for the convenience of the recovery of the water insoluble products. A similar two -phase system was used for hydrolysis of the complex phospholipids by phosp holipase C form Bacillus cereus, which released both lysophospholipids. Onl y trace amounts of phosphatidic acid (PA) were detected showing that the en zyme is highly selective for the release of the diacylglycerol and 1-lauroy l-rac-glycerophosphate and 1-lauroyl-dihydroxyacetonephosphate. (C) 2000 El sevier Science Ireland Ltd. All rights reserved.