An improved ELISA for the determination of southern bean mosaic virus withlinear sweep voltammetry detection based on new system of PAP-H2O2-HRP

An improved enzyme-linked immunosorbent assay (ELISA) for the determination of southern bean mosaic virus (SBMV) with linear sweep voltammetry based o n a new system of p-aminophenol (PAP)-H2O2-horseradish peroxidase (HRP) has firstly been developed. The enzymatic product 3-[(4-hydroxyphenyl) amino]- 1-(2-amino-5-hydroxyphenyl)-6-[(4-hydroxyphenyl) imino]-2,4-cyclohexadiene- 1-one, produced from the oxidation of PAP with H2O2 catalyzed by HRP, yield ed a sensitive linear sweep voltammetric response at -0.45 V (vs. SCE) in B ritton-Robinson (BR) buffer solution. Based on the voltammetric peak, HRP c an be measured with a detection limit of 0.4 mU/L and a linear range of 1.0 -1.0 x 10(2) mU/L, The detection limit for the SBMV is 8.0 ng/mL and the hi ghest dilution ratio for the detection of infected leaf sap is 1: 1.5 x 10( 5).