Redox reactions of cytochrome c facilitated by silver-imidazole complex

An imidazole modified silver electrode is prepared by immersing the substra te silver electrode in a 2% imidazole solution of ethanol at 50 degrees C f or 10 min. The modified electrode is then swept in a cytochrome c solution and the modified layer takes off because the modified electrode is very uns table. Although the amount of the silver-imidazole complex is very snail co mpared with the amount of cytochrome c in the protein solution, it greatly facilitates redox reactions involving the biomacromolecules.