Analysis of flavonols of Sedum telephium L. leaves by capillary electrophoresis and HPLC-mass spectrometry

Flavonol glycosides of Sedum telephium L. were analysed by MEKC. Baseline s eparation was achieved within 14 min using a fused silica capillary and a b orate/phosphate buffer solution (35 mM, pH 5.8) containing 35 mM SDS and 4 % MeOH. The applied voltage was 25 kV and the thermostating temperature was kept constant at 40 degrees C. Injection was performed via the pressure mo de for 3 s, the detection wavelength was 205 nm. The optimized MEKC method was used for the quantitative determination of flavonol glycosides in extra cts of S. telephium leaves. Analysis was also performed by HPLC-MS using an electrospray ionization (ES I) interface. The good agreement between the quantitative CE results and th ose obtained by LC clearly demonstrated the applicability of the methods pr esented.