Cm. Baecher-allan et al., Generation of a polyclonal Fab phage display library to the protozoan parasite Cryptosporidium parvum, COMB CHEM H, 2(6), 1999, pp. 319-325
Citations number
15
Categorie Soggetti
Chemistry & Analysis
Journal title
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
We had developed a technology for creation of recombinant polyclonal antibo
dy libraries, standardized perpetual mixtures of polyclonal whole antibodie
s for which the genes are available and can be altered as desired. We repor
t here the first phase of generating a polyclonal antibody library to Crypt
osporidium parvum, a protozoan parasite that causes severe disease in AIDS
patients, for which there is no effective treatment. BALB/c mice, immunized
by neonatal oral infection with oocysts followed by intraperitoneal immuni
zation with a sporozoite/oocyst preparation of C. parvum, were used for con
struction of a Fab phage display library in a specially-designed bidirectio
nal vector. This library was selected for reactivity to an oocyst/sporozoit
e preparation, and was shown to be antigen-specific and diverse. Following
mass transfer of the selected variable region gene pairs to appropriate mam
malian expression vectors, such anti-C. parvum Fab phage display libraries
could be used to develop chimeric polyclonal antibody libraries, with mouse
variable regions and human constant I regions, for passive immunotherapy o
f C. parvum infection.