The effect of DNA/gold particle preparation technique, and particle bombardment device, on the transformation of barley (Hordeum vulgare)

Immature embryos of the spring barley variety Golden Promise, were bombarde d with three different particle delivery systems and both transient and sta ble transformation examined. In addition, a range of techniques for the pre paration of the DNA coated gold particles was examined. Fertile transgenic barley plants were obtained using three particle preparation techniques whi ch differed in the amount of gold and DNA used for each bombardment. Howeve r, only one of the particle delivery systems, the PDS 1000/He device, appea red to be effective in yielding transformed barley plants.