The structure of the carbohydrate backbone of core-lipid A region of the lipopolysaccharides from Proteus mirabilis wild-type strain S1959 (serotype O3) and its Ra mutant R110/1959

The following structure of core-lipid A region of the lipopolysaccharide (L PS) from Proteus mirabilis strain 1959 (serotype O3) and its rough mutant R 110/1959 (Proteus type II core) was determined using NMR and chemical analy sis of the core oligosaccharide, obtained by mild acid hydrolysis of LPS, a nd of the products of alkaline deacylation of the LPS: [GRAPHICS] Incomplete substitutions are indicated by italics. All sugars are in pyrano se form, alpha-Hep is the residue lglycero-alpha-dmanno-Hep, alpha-dd-Hep i s the residue dglycero-alpha-dmanno-Hep. The differences with the previousl y reported structures are discussed.