COMMENT ON MOBILITY-SHIFT COMPUTATIONS FEATURING CAGE EFFECTS

Theoretical mobility-shift patterns are computed by solution of conser vation equations for electrophoresis coupled with chemical reaction, T he chemical reaction term is often formulated in terms of dissociation of the protein-DNA complex in a gel cage. This formulation assumes th at once the dissociated protein escapes the cage, it goes down a sink and is totally lost, This implies that the concentration of the escape d protein is too low to affect significantly the rates of protein-DNA association along its migration pathway.