Characterization of alpha(1)-adrenoceptor subtypes in the eye

The presence of the alpha(1)-adrenoceptor subtypes in various parts of the pig and rabbit eyes was investigated using [H-3]-prazosin radioligand bindi ng. The characterization of the subtypes was achieved by performing competi tion experiments with various subtype selective drugs. In the pig retina, b oth alpha(1A)- and alpha(1B)-adrenoceptors were detected and the proportion of sites was 70% alpha(1A)- and 30% alpha(1B)-adrenoceptors, respectively. In the pig iris, ciliary body and choroid, which are melanin-rich tissues, the non-specific binding of [H-3]-prazosin was too high to detect any of t he alpha(1)-adrenoceptor subtypes. However, in the albino rabbit iris, cili ary body and retina both alpha(1A)- and alpha(1B)-adrenoceptors were detect ed. The proportion of sites in the iris was 60 % alpha(1A)- and 40% alpha(1 B)-adrenoceptors, respectively. In the ciliary body and rabbit retina the p roportion of sites were 70% alpha(1A)- and 30% alpha(1B)-adrenoceptors. Onl y the alpha(1A)-adrenoceptor subtype was detected in the rabbit choroid. (C ) 2000 Academic Press.