Levels of 8-hydroxydeoxyguanosine as a marker of DNA damage in human leukocytes

We measured 8-hydroxy-2-deoxyguanosine (8-OHdG) levels in human leukocytes from healthy donors to evaluate oxidative DNA damage and its correlation wi th smoking, physical exercise, and alcohol consumption. A significant incre ase in oxidative DNA damage was induced by cigarette smoke, with the mean l evel of 8-OHdG being significantly higher in smokers (33.1 +/- 10.6 per 10( 6) 2-deoxyguanosine (dG) [mean +/- SE], n = 16) compared with nonsmokers (1 5.3 +/- 1.8 per 10(6) dG, n = 31) and former smokers (17.8 +/- 1.5 per 10(6 ) dG, n = 9). The highest values were observed after smoking more than 10 c igarettes per day (41.8 +/- 17.1 per 10(6) dG, n = 9). A large interindivid ual variation in 8-OHdG levels was observed in all analyzed groups. We also observed a correlation between 8-OHdG levels and age in nonsmokers and for mer smokers, Neither frequency of physical exercise nor alcohol drinking si gnificantly modified 8-OHdG levels in leukocytes. (C) 2000 Elsevier Science Inc.