The temperature dependence of delayed millisecond fluorescence (DF) inducti
on curve transients was investigated. The transients were obtained by keepi
ng intact maize leaf segments in the dark for 30 to 300 s before illuminati
on. The temperature dependence of DF induction revealed abrupt changes in a
ctivation energy of the recombination process. Those changes highly overcom
e the lowest energy barriers for certain types of chemical reactions, imply
ing significant possible alterations in the DF mechanism itself. On the oth
er hand, the particular transients responses expressed some specifities. Bo
th the electrochemical gradient controlled changes, and the temperature ind
uced changes contibute to it.