A physical map of the 20q12-q13.1 region associated with Type 2 diabetes

Several recent genetic studies have suggested Linkage of Type 2 diabetes (n on-insulin-dependent diabetes mellitus) susceptibility to a region of chrom osome 20q12-q13.1, To facilitate the identification and cloning of a diabet es susceptibility gene(s) in this region, me have constructed correlated ra diation hybrid and YAC/BAC contig physical maps of the region. A high-resol ution radiation hybrid map encompassing 9.5 Mb between the PLC and the CEBP B genes was constructed using 68 markers: 25 polymorphic markers, 15 known genes, 21 ESTs, and 7 random genomic sequences. The physical order of the p olymorphic markers within this radiation hybrid map is consistent with publ ished genetic maps. A YAC/BAC contig that gives continuous coverage between PLC and CEBPB was also constructed. This contig was constructed from 24 YA Cs, 34 BACs, and 1 P1 phage clone onto which 71 markers were mapped: 23 pol ymorphic markers, 12 genes, 24 ESTs, and 12 random genomic sequences. The r adiation hybrid map and YAC/BAC physical map enable precise mapping of newl y identified transcribed sequences and polymorphic markers that will aid in linkage and Linkage disequilibrium studies and facilitate identification a nd cloning of candidate Type 2 diabetes susceptibility genes residing in 20 q12-q13.1. (C) 1999 Academic Press.