Immobilization of alpha-amylase on porous silica

Citation
Hm. Mody et al., Immobilization of alpha-amylase on porous silica, I J CHEM A, 38(11), 1999, pp. 1200-1202
Citations number
15
Categorie Soggetti
Chemistry
Journal title
INDIAN JOURNAL OF CHEMISTRY SECTION A-INORGANIC BIO-INORGANIC PHYSICAL THEORETICAL & ANALYTICAL CHEMISTRY
ISSN journal
03764710 → ACNP
Volume
38
Issue
11
Year of publication
1999
Pages
1200 - 1202
Database
ISI
SICI code
0376-4710(199911)38:11<1200:IOAOPS>2.0.ZU;2-2
Abstract
Porous silica has been synthesized by hydrolysis of sodium silicate with am monium chloride in the presence of sodium lauryl sulphate and the resultant gel hydrothermally treated. Surface of the silica, thus prepared, has been functionalized by silylation using 3-aminopropyl triethoxysilane. This mat erial has been used as a support for immobilization of alpha-amylase. Starc h hydrolyzing activity of immobilized enzyme remains unchanged even after 1 0 days, thereby showing that surface derivatised silica is a potential supp ort for the immobilization of enzymes.