Identification of the operon for the sorbitol (glucitol) phosphoenolpyruvate : sugar phosphotransferase system in Streptococcus mutans

Transposon mutagenesis and marker rescue were used to isolate and identify an 8.5-kb contiguous region containing six open reading frames constituting the operon for the sorbitol P-enolpyruvate phosphotransferase transport sy stem (PTS) of Streptococcus mutans LT11. The first gene, srlD, codes for so rbitol-6-phosphate dehydrogenase, followed downstream by srlR, coding for a transcriptional regulator; srlM, coding for a putative activator; and the srlA, srlE, and srlB genes, coding for the EIIC, EIIBC, and EIIA components of the sorbitol PTS, respectively. Among all sorbitol PTS operons characte rized to date, the srlD gene is found after the genes coding for the EII co mponents; thus, the location of the gene in S. mutans is unique. The SrlR p rotein is similar to several transcriptional regulators found in Bacillus s pp. that contain PTS regulator domains (J. Stulke, M. Arnaud, G. Rapoport, and I. Martin-Verstraete, Mel. Microbiol. 28:865-874, 1998), and its gene o verlaps the srlM gene by 1 bp. The arrangement of these two regulatory gene s is unique, having not been reported for other bacteria.