Comparative analysis of antibody responses against HSP60, invariant surface glycoprotein 70, and variant surface glycoprotein reveals a complex antigen-specific pattern of immunoglobulin isotype switching during infection byTrypanosoma brucei

During Trypanosoma brucei infections, the response against the variant surf ace glycoprotein (VSG) of the parasite represents a major interaction betwe en the mammalian host immune system and the parasite surface. Since immune recognition of other parasite derived factors also occurs, we examined the humoral host response against trypanosome heat shock protein 60 (HSP60), a conserved antigen with an autoimmune character. During experimental T. bruc ei infection in BALB/c mice, the anti-HSP60 response was induced when paras ites differentiated into stumpy forms. This response was characterized by a stage-specific immunoglobulin isotype switching as well as by the inductio n of an autoimmune response. Specific recognition of trypanosome HSP60 was found to occur during the entire course of infection. Immunoglobulin G2a (I gG2a) and IgG2b antibodies, induced mainly in a T-cell-independent manner, were observed during the first peak of parasitemia, whereas IgG1 and IgG3 a ntibodies were found at the end of the infection, due to a specific T-cell- mediated response. Comparative analysis of the kinetics of anti-HSP60, anti -invariant surface glycoprotein 70 (ISG70), and anti-VSG antibody responses indicated that the three trypanosome antigens give rise to specific and in dependent patterns of immunoglobulin isotype switching.