Identification of genes differentially expressed in canine vasospastic cerebral arteries after subarachnoid hemorrhage

Citation
H. Onda et al., Identification of genes differentially expressed in canine vasospastic cerebral arteries after subarachnoid hemorrhage, J CEREBR B, 19(11), 1999, pp. 1279-1288
Citations number
33
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
ISSN journal
0271678X → ACNP
Volume
19
Issue
11
Year of publication
1999
Pages
1279 - 1288
Database
ISI
SICI code
0271-678X(199911)19:11<1279:IOGDEI>2.0.ZU;2-3
Abstract
To understand the molecular processes of continuous vasospasm of cerebral a rteries after subarachnoid hemorrhage, mRNA differential display and screen ing of cDNA expression array were performed to identify genes that are diff erentially expressed in vasospastic arteries of canine two-hemorrhage model s. The expression levels of Is genes were found to be upregulated, and thos e of two genes to be downregulated. Of these, 12 represent known genes or h omologues of genes characterized previously, and the other eight genes are not related to any sequences in the databases. The known genes include five upregulated inflammation-related genes encoding monocyte chemotactic prote in-1, cystatin B, inter-alpha-trypsin inhibitor family heavy chain-related protein, serum amyloid A protein, and glycoprotein 130, suggesting that inf lammatory reaction may be involved in the development of cerebral vasospasm . The upregulation of three known genes encoding stress-related proteins of vascular endothelial growth factor, BiP protein, and growth-arrest and DNA -damage-inducible protein may be involved in possible cell survival in the damaged arteries. A full-length cDNA for the unknown clone DVS 27, whose ex pression was most highly upregulated, was isolated from the cerebral artery cDNA library by hybridization. Char acterization of these genes should hel p to clarify the molecular mechanism of continuous cerebral vasospasm after subarachnoid hemorrhage.