H. Onda et al., Identification of genes differentially expressed in canine vasospastic cerebral arteries after subarachnoid hemorrhage, J CEREBR B, 19(11), 1999, pp. 1279-1288
To understand the molecular processes of continuous vasospasm of cerebral a
rteries after subarachnoid hemorrhage, mRNA differential display and screen
ing of cDNA expression array were performed to identify genes that are diff
erentially expressed in vasospastic arteries of canine two-hemorrhage model
s. The expression levels of Is genes were found to be upregulated, and thos
e of two genes to be downregulated. Of these, 12 represent known genes or h
omologues of genes characterized previously, and the other eight genes are
not related to any sequences in the databases. The known genes include five
upregulated inflammation-related genes encoding monocyte chemotactic prote
in-1, cystatin B, inter-alpha-trypsin inhibitor family heavy chain-related
protein, serum amyloid A protein, and glycoprotein 130, suggesting that inf
lammatory reaction may be involved in the development of cerebral vasospasm
. The upregulation of three known genes encoding stress-related proteins of
vascular endothelial growth factor, BiP protein, and growth-arrest and DNA
-damage-inducible protein may be involved in possible cell survival in the
damaged arteries. A full-length cDNA for the unknown clone DVS 27, whose ex
pression was most highly upregulated, was isolated from the cerebral artery
cDNA library by hybridization. Char acterization of these genes should hel
p to clarify the molecular mechanism of continuous cerebral vasospasm after
subarachnoid hemorrhage.