Synthesis of a silica-bonded bovine serum albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers

A novel method of synthesizing protein chiral stationary phase (protein-CSP ) is proposed with 2,4,6-trichloro-1,3,5-triazine as the activator. The bov ine serum albumin (BSA) based chiral columns (150x4.6 mm I.D.) were prepare d successfully within 8 h. With tryptophan as the probe solute, it was obse rved that the BSA immobilized by this method had a better ability to distin guish enantiomers than that activated by glutaric dialdehyde. This may be d ue to the well-maintained BSA conformation and the larger amount of BSA imm obilized on the silica gel. The BSA-CSP prepared by this method was relativ ely stable under experimental conditions, and the resolution of 13 chiral c ompounds was achieved. The coupling reaction in this method is mild, reliab le and reproducible; it is also suitable for the immobilization of various biopolymers in the preparation of bioreactor, biosensor and affinity chroma tography columns. (C) 2000 Elsevier Science B.V. All rights reserved.